La transpiración vegetal consiste en la pérdida de agua en forma de vapor que se produce en las plantas. A las hojas de éstas llega gran cantidad de agua absorbida por las raíces, pero sólo una pequeña parte se utiliza en la fotosíntesis. Su principal función es eliminar en forma de vapor el agua que no es utilizada por las plantas. Además, el agua transpirada permite el enfriamiento de la planta, debido al elevado calor de vaporización del agua (para evaporarse necesita consumir muchas calorías). Normalmente es muy difícil distinguir la transpiración de la evaporación proveniente del suelo por lo que al fenómeno completo se le denomina "evapotranspiración", siendo éste un parámetro importante en el diseño de la técnicas de regadío que se utilizarán.

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Transpiration is the process of water movement through a plant and its evaporation from aerial parts, such as leaves, stems and flowers. Water is necessary for plants but only a small amount of water taken up by the roots is used for growth and metabolism. The remaining 97–99.5% is lost by transpiration and guttation. Leaf surfaces are dotted with pores called stomata, and in most plants they are more numerous on the undersides of the foliage. The stomata are bordered by guard cells and their stomatal accessory cells (together known as stomatal complex) that open and close the pore. Transpiration occurs through the stomatal apertures, and can be thought of as a necessary "cost" associated with the opening of the stomata to allow the diffusion of carbon dioxide gas from the air for photosynthesis. Transpiration also cools plants, changes osmotic pressure of cells, and enables mass flow of mineral nutrients and water from roots to shoots. Two major factors influence the rate of water flow from the soil to the roots: the hydraulic conductivity of the soil and the magnitude of the pressure gradient through the soil. Both of these factors influence the rate of bulk flow of water moving from the roots to the stomatal pores in the leaves via the xylem. Mass flow of liquid water from the roots to the leaves is driven in part by capillary action, but primarily driven by water potential differences. If the water potential in the ambient air is lower than the water potential in the leaf airspace of the stomatal pore, water vapor will travel down the gradient and move from the leaf airspace to the atmosphere. This movement lowers the water potential in the leaf airspace and causes evaporation from the mesophyll cell wall menisci of liquid water. This evaporation increases the tension on the menisci surface and increases its radius. Because of the cohesive properties of water, the tension travels through the leaf cells to the leaf and stem xylem where a momentary negative pressure is created as water is pulled up the xylem from the roots. In taller plants and trees, the force of gravity can only be overcome by the decrease in hydrostatic (water) pressure in the upper parts of the plants due to the diffusion of water out of stomata into the atmosphere. Water is absorbed at the roots by osmosis, and any dissolved mineral nutrients travel with it through the xylem. The Cohesion-tension theory explains how leaves pull water through the xylem. Water molecules stick together, or exhibit cohesion. As a water molecule evaporates from the surface of the leaf, it pulls on the adjacent water molecule, creating a continuous flow of water through the plant.

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