Un subfósil es un remanente o resto biológico cuyo proceso de fosilización no está completo, quizás por falta de más tiempo o debido a la forma en que fueron sepultados, no óptimas para tal proceso de fosilización. Los subfósiles de antes del Mesozoico son excepcionalmente raros, usualmente en un estado avanzado de deterioro y consecuentemente de origen controvertido. La mayor parte de material subfósil se encuentra en depósitos del Cuaternario. La principal importancia de los restos subfósiles frente a los fósiles es que aún contienen extractos orgánicos, que podrán usarse para su datación por radiocarbono o extraerse y secuenciar ADN, proteínas u otras biomoléculas. Además, los cocientes isotópicos pueden dar mucha información de las condiciones ecológicas de la época en que vivieron.

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A subfossil (as opposed to a fossil) is a bone or other part of an organism that has not fully fossilized. This may be because not enough time has elapsed since the animal died, or because the conditions in which the remains were deposited were not optimal for fossilization. For remains such as molluscan seashells, which frequently do not change their chemical composition over geological time, and may occasionally even retain such features as the original color markings for millions of years, the label "subfossil" is applied to shells that are understood to be thousands of years old, but are of Holocene age, and therefore are not old enough to be from the Pleistocene epoch. Unfossilized or partially fossilized remains can include bones, exoskeletons, nests, skin imprints, or fecal deposits. Subfossils of vertebrates are often found in caves or other shelters, where the remains have been preserved for thousands of years. The main importance of these vertebrate subfossil (versus fully fossilized) remains is that they contain organic material, which can be used for radiocarbon dating or the extraction and sequencing of DNA, protein, or other biomolecules. Additionally, isotope ratios can provide information about the ecological conditions under which extinct animals lived. Subfossils are useful for studying the evolutionary history of an environment and can be important to studies in paleoclimatology. Subfossils are also often found in depositionary environments, such as lake sediments, oceanic sediments, and soils. Once deposited, physical and chemical weathering may alter the state of preservation, and small subfossils can also be ingested by living organisms. Subfossil remains that date from the Mesozoic are exceptionally rare, are usually in an advanced state of decay, and are consequently much disputed. The vast bulk of subfossil material comes from Quaternary sediments, including many subfossilized chironomid head capsules, ostracod carapaces, diatoms, and foraminifera.

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